FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18033202
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAACG97800.042.03425620-21
CAAACGA97550.039.050320-21
CGGGGAT109950.033.9148148
TAGTAGC178550.033.5177577
GTAGCAA184950.032.6052939
AAACGAG134000.030.97880422-23
CCCCGTT51150.030.961472
GGTGTGC1374250.030.2439466
AGTAGCA203150.029.7743078
TCGGGGA128500.029.3037917
CGGTGTG455900.029.113325
AGTCGGT138050.028.88386286-87
TCAGTCG139400.028.75151384-85
TGTGCTC2452550.028.5169498
GTGTGCT2475600.028.4122247
AAGGCCG146350.028.1834136-37
GTGCTCT2499800.028.016379
ACGAGAA149300.027.87645124-25
CCCCCGT42100.027.5055981
CCCGTTA22800.027.5001183
Produced by FastQC (version 0.11.5)