FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908028
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC237160.1705202204079543No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG169910.12216685212310473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA47850.049.1328284
GCGACAT50100.046.9257555
CTGCGAC66850.035.5946583
GCTGCGA71050.033.3593642
CGACATC83900.028.5872026
CAAGCTT244400.027.8889372
AAGCTTG253850.027.0357973
TTGGGCT264450.025.9158237
TAGCCCC45950.025.7603031
AGCTTGG273450.025.4627064
GGTCGAC239300.025.36464716-17
CTTGGGC279450.024.7796786
GCTTGGG285700.024.6699055
TAGCCCA53500.024.6330641
TCGACCC255250.023.55638518-19
GTGTGCT136850.023.391468
TAGCCGC18300.023.0447041
TAGGCCC42800.022.9535371
GACCCGT253700.022.84851620-21
GGCTGCG108350.022.822351
Produced by FastQC (version 0.11.5)