FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908028
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153670.11049014281535816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAA170350.040.6814659
TAGTAGC181150.038.216277
TTCCGAC46250.037.2973568
AGTAGCA192950.036.0537348
TCCGACC48200.035.699929
AGTACGA63750.034.386999
CAGTACG64900.034.204638
TCGCGCG79700.033.1718257
CGGGGAT109200.032.8019528
CGAGAAC138250.032.32119424-25
CAAACGA140200.031.84031520-21
AATACCG16850.031.6072888
ACGAGAA140700.031.60707724-25
AAACGAG141450.031.47030322-23
CGAAGTG141250.030.97514540-41
TCAGTCG112200.030.81486184-85
TCGGGGA118200.030.4607627
CGGTGAA72700.030.1301925
TCACCCC157350.029.9908479
GTACGGT13300.029.738152
Produced by FastQC (version 0.11.5)