FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19951222
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC1511310.7575024727808652No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1092220.5474451640105052No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC471240.23619605856723966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA183200.083.70534
GCGACAT185850.082.562875
CTGCGAC199850.076.826653
CGACATC204600.075.0430456
GCTGCGA211300.072.88832
GGCTGCG239000.064.9801561
GACATCT300000.051.3693247
ACATCTG303550.050.739568
CATCTGT338450.045.4793789
ATTCGGC202750.037.8759838-39
TTCGGCT206600.037.11269440-41
TTGATCG207350.037.03248622-23
TGATTCG207900.036.96062536-37
TCGGCTG211300.036.3883340-41
TGATCGC211900.036.2597324-25
TCGCCAG218950.035.25710726-27
GATCGCC222650.034.5730624-25
CGCCAGG234350.032.911128-29
ATCGCCA237300.032.58078426-27
CGGCTGA240700.032.12125442-43
Produced by FastQC (version 0.11.5)