FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17605267
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG360240.2046205831470775No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG182790.10382688317081475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG45300.046.127575
CGGGGAT232450.042.190198
TCGGGGA241650.040.7222027
CTGTCGG51450.039.506134
TTCCGAC57800.037.714288
TCTGTCG56350.036.152323
GTCGGGG62500.035.3339846
TCCGACC62350.035.038249
ATCGGGG175650.033.6891146
TCTATCG131200.033.371053
CTATCGG140600.032.324574
TATCGGG148850.032.0325135
GTTCCGT28150.030.3621391
GTCCCGT28100.029.7402481
GGGGATG333850.029.54659
TCAGTCG100600.029.26448284-85
CCCCGTT50200.028.9444852
ATAGCGC100950.028.8482794-95
GTAGCAA152700.028.644529
AGTCGGT102700.028.5515886-87
Produced by FastQC (version 0.11.5)