FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25078301
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC451920.18020359513190307No Hit
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT451670.18010390735799844No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG431190.171937484919732No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC414940.16545777961593172No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC315460.12579002062380543No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC289540.11545439222537443No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG264890.10562517771837894No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC252720.10077237688470204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT66000.063.484415
TGCGACA66800.062.5772674
CTGCGAC75250.055.8028223
GCTGCGA85950.049.1874732
ACCCGAC117000.039.5036623
CCGACTC118800.038.9079935
CCCGACT120550.038.537374
GGCTGCG111050.038.514711
CGACTCC124000.037.3487436
AACCCGA126350.036.9563142
GAACCCG159750.030.7299671
GACTCCC159900.029.2010277
TCGATCG86250.027.28690516-17
CTAGGCG196300.024.8509647
ACTCCCT187500.024.80138
GGTGTGC156150.024.7614157
TGATCGC84450.024.71854224-25
TAGGCGG200450.024.3127658
TTCGATC97200.024.285914-15
TCGGCCG98600.023.8691220-21
Produced by FastQC (version 0.11.5)