FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23706501
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC555820.2344588937861391No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC303960.12821799387433852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA54000.055.0525054
GCGACAT54750.054.124885
CTGCGAC61850.048.2188033
CGACATC70900.041.9299776
GCTGCGA72350.041.2209132
CTAGGCG164050.034.9404457
TAGGCGG170150.033.6618168
GGCTGCG99000.031.1033631
GCTAGGC194750.029.7738296
GGCTAGG222150.026.6145745
TGGCTAG239750.024.9183084
AGGCGGG248550.023.3687159
CGGTCGA121900.023.36063268-69
CCGATAG84450.022.72630990-91
TTGATCG66850.022.39046522-23
TCGGTCG127000.022.14201468-69
GGCTGGC274000.022.0766531
CGCTCGG135200.021.6951764-65
CGACCAT130950.021.3668782-83
TGATCGC70750.021.12268424-25
Produced by FastQC (version 0.11.5)