FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23706501
Sequences flagged as poor quality0
Sequence length101
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCGT42150.034.0901031
CGGGGAT132250.033.6221588
TCCGACC36800.033.0665479
CCCCGTT56100.033.036842
GTCCCGT34300.032.286241
TCGGGGA143750.031.1869747
CCCCGAA54850.031.1199262
GCCCCGT40050.029.0220071
TTCCGAC42250.029.0177068
TGTCGGG34500.028.6509575
CCCGTTA28400.028.357963
CCGTTAA26500.028.3186264
CCGATCG56200.028.06772686-87
CCGTTTA37350.027.8105454
GTTCCGT32200.027.4282231
CCCGTTT76600.027.419823
CGATCGG56500.027.19037886-87
TCCCCGT58700.026.5055661
ATCGGGG101900.026.0829646
TATCGGG81500.025.8288295
Produced by FastQC (version 0.11.5)