FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21774921
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG7257203.332824950317845No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA3467341.5923548011953752No Hit
TGGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3336051.5320606674072434No Hit
TGGTGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG3106601.4266871507823151No Hit
TGGTGCTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG2170550.9968118828077493No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1762900.8096011002749447No Hit
TGGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1740480.7993048516685778No Hit
TGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1723160.7913507470360053No Hit
TGGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1588580.7295457007628179No Hit
TGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1382550.6349276766606868No Hit
TGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1333920.6125946450046822No Hit
TTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1312960.6029688925163035No Hit
TGGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA1235210.567262678013849No Hit
GGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG1178310.5411316991689661No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA1097560.5040477529172207No Hit
TGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA902770.414591630435766No Hit
GTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA864320.3969337018490216No Hit
GGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGT822690.37781537760802897No Hit
CTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA821900.37745257491404904No Hit
ATGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA758380.34828140134239755No Hit
GGTGGCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA732790.3365293495209466No Hit
TGGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA729470.33500465971839805No Hit
TGTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA692790.3181595928637353No Hit
TTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGG681880.3131492417354809No Hit
TGTGGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG638210.29309405990497045No Hit
AGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG623010.2861135523752302No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG612090.2810986088078115No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG582010.2672845518015886No Hit
TTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC559080.2567540887978423No Hit
TGATTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC536310.24629710482072473No Hit
GTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTAC531390.24403762475188776No Hit
GTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG490270.2251535149082745No Hit
AGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA477440.21926141546047398No Hit
TGCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA467780.2148251192277575No Hit
TGGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA458780.21069192397988493No Hit
TTGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG418800.19233135220100225No Hit
TGGGGATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG417400.19168841071799986No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA415590.19085717922926104No Hit
AAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAA400840.18408333146191438No Hit
TGGTCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG383700.17621189073429933No Hit
TAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG376760.17302473795427317No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC371840.17076525788543617No Hit
TGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA371590.17065044690632863No Hit
CGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG371220.17048052665724941No Hit
GGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGC370590.17019120298989834No Hit
GATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCA362970.16669176434669958No Hit
GCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG358840.1647950869718425No Hit
ATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGG347890.15976636608693093No Hit
TTGTGGAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC346260.15901779850314957No Hit
TAGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA344590.15825086116271098No Hit
TGGTTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA338450.15543110351582906No Hit
TTGGTAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA334160.15346094711434316No Hit
CCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG331990.15246438781568944No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT327250.1502875716518099No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC311360.14299018581973272No Hit
GGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCC306160.14060211745429524No Hit
TGGCAGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG295650.13577546389261297No Hit
GGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTAA295010.13548154778609758No Hit
GGTGTTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA294110.13506822826131035No Hit
TGAGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA288850.13265260526088707No Hit
TTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGA288170.13234031939771446No Hit
TGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG287430.13200047889955605No Hit
TGGCCGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG284910.13084318423015176No Hit
TTGGTTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA283070.12999817542392No Hit
TGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAG282120.12956189370331125No Hit
TTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTG278770.12802342658326982No Hit
GGTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA276020.12676050581308654No Hit
TTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGC273330.12552513967788909No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC272190.12500160161315854No Hit
TGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG263680.12109343588433685No Hit
TGCTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG262390.12050101123214178No Hit
GGGGTGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA259250.1190589853345507No Hit
TGGTAATAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG257990.11848033799984854No Hit
TTGTGTAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCC249200.11444358397442636No Hit
GGTTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG247050.11345620955410125No Hit
CTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGT246160.11304748246847829No Hit
TGGTTTTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC245260.11263416294369105No Hit
TGGATGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGA244910.11247342757294045No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA244160.11212899463561773No Hit
GTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT243000.1115962716925586No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA240500.1104481619014829No Hit
GGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGC239070.1097914431009876No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG236340.10853770720913292No Hit
CATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGG236090.10842289623002536No Hit
GCCCGGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA232670.10685228203583379No Hit
CCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGT228630.10499693661345545No Hit
TGGCTGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG228230.10481323904688335No Hit
TCGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG222340.10210829237910898No Hit
TGATTCTCAGTACGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTC222040.1019705192041799No Hit
TGGTCAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA221560.10175008212429336No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA220400.10121735918123423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTGTT397750.088.3704151
GGTGCTA269050.087.4323962
GTGCTAG273950.087.274343
TGCTAGT284350.086.823764
TGGTGCT264500.085.376481
TGATAGT447000.084.643854
GGTGTTA456650.083.687482
TTCGACG14900.082.6213761
GATAGTA543450.082.2863165
GGTGATA440800.078.814162
GTGATAG458350.078.679713
GTACGGT23450.078.646032
GCTAGTA324050.078.049685
TGGTGAT416150.077.9185261
CGCGATA6350.077.848373
TGGGGTA227450.075.606921
TGGTTAG152450.074.173021
ATAGTAG664900.074.1166156
GTGGTAC37700.073.505044
CGACCTA15950.073.3083341
Produced by FastQC (version 0.11.5)