FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21635894
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC363990.1682343239433508No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT247000.1141621418555665No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT243440.11251672798914618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA46600.040.9732254
GCGACAT52650.036.178225
CTGCGAC61600.031.4586093
ACTCGGG182750.031.0954931
CTAGGCG131800.030.3097087
TAGGCGG137250.029.0023278
CTCGGGA201350.028.3067262
GCTAGGC148450.026.9741976
GCTGCGA72400.026.5034752
TCGGGAG218300.026.174113
GGCTAGG162850.025.2015445
CGACATC76600.024.928636
TGGCTAG173100.024.036274
GGTCGAC49100.019.68581816-17
ACGGACC41550.019.6615354
CGGTCGA109700.018.53347668-69
GGTGTGC148200.018.1733937
GCGCGGT41400.018.1283346
CTGGCTA237700.018.0234283
CGGGAGG335350.017.8881344
Produced by FastQC (version 0.11.5)