FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12378787
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG277770.22439193759453166No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA253590.20485852127514595No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175660.14190404924165834No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC159700.12901102507055012Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG154460.12477797703442187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC49750.045.757238
TCCGACC50250.045.3160639
TGTCGGG42200.040.4129645
TCTGTCG42500.038.667713
CGGGGAT200650.038.2167788
TCGGGGA205700.037.5034187
CCGTTTC62400.034.576494
TTTCCGA67250.034.406917
TCGCGCG58050.034.339557
TCACCCC167300.032.9427389
CTGTCGG50850.032.8879974
CGTTTCC61250.032.612525
TCCGTTT63900.032.4237943
CTCCGTT54500.032.0507662
TCGATCG5250.031.3921243
ATCGGGG149250.030.8718876
CGCGCGT65300.030.674788
ACGGTCG7700.030.5917195
GTTTCCG66100.030.5769736
CCGTTTA19250.030.3452664
Produced by FastQC (version 0.11.5)