FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18523160
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC609760.32918789234666224No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC440050.2375674560928049No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC298200.16098765005539012No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT270900.14624934406440368No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT270680.1461305738329745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA68600.063.696874
GCGACAT72400.060.4966245
CTGCGAC82200.053.5047533
CGACATC91450.047.7387056
GCTGCGA97050.045.7582362
GGCTGCG121550.037.0614431
ACTCGGG185150.031.568241
CTAGGCG230350.030.6250657
CTCGGGA201600.029.2371122
TAGGCGG248700.028.7856668
TGGCTAG249400.028.7564264
TCGGGAG210800.027.961113
GCTAGGC255600.027.5997056
GACATCT163300.027.2578897
GGCTAGG267500.026.7981175
TTGATCG84800.026.072922-23
TGATCGC88700.024.92594524-25
ACATCTG180150.024.866598
CTGGCTA296050.024.578023
GGCTGGC301850.023.897341
Produced by FastQC (version 0.11.5)