FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24377476
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG540610.22176619105275705No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG283220.1161810189045002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC69800.047.6925669
CGGGGAT302050.045.2479748
TCGGGGA318750.043.0117767
TTCCGAC77700.042.782318
TGTCGGG65700.041.346215
TCTATCG168500.037.2024843
ATCGGGG228850.036.2104156
CTGTCGG73950.035.8987664
TATCGGG188400.035.8950165
CTATCGG183400.035.190554
CCGTTAA26500.034.7665634
GTCGGGG82150.034.395246
TCTGTCG77600.034.0260053
GTTCCGT36550.033.125711
CCGTTTC109850.032.813134
CCGTTTA41200.032.3903054
GTCCCGT42200.032.178511
CCCCGTT74350.031.9301172
CCGATCG122700.031.49179586-87
CGATCGG123350.031.2680586-87
Produced by FastQC (version 0.11.5)