FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20801727
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC348750.16765434908361215No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC291690.14022393429160954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA49500.056.0414854
GCGACAT49950.055.7313545
CTGCGAC55800.050.224983
GCTGCGA67300.041.8544432
GGCTGCG87750.033.4154051
CGACATC84600.033.070096
ACCCGAC42900.029.5635053
ACTCGGG109200.026.8951871
CCGACTC47200.026.771775
CCCGACT47950.026.7471264
CTAGGCG151000.025.6686887
CGACTCC48600.025.6068126
TAGGCGG155150.025.0127128
CTCGGGA120850.024.8018842
TCGATCG27950.023.02634416-17
AACCCGA59050.022.2823932
GCTAGGC179500.022.0959426
TCGGGAG136450.021.722663
TTGATCG65400.021.60588522-23
TGATCGC66000.021.37325324-25
Produced by FastQC (version 0.11.5)