FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18523929
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC69500.045.4864968
TCCGACC71200.044.3361859
TTTCCGA89350.035.688437
CCCGTTA24400.035.4451073
GTTTCCG79500.034.758346
GTCCCGT26550.034.2998051
ATCGCGA7100.034.00493218-19
CCCCGTT51650.033.9339072
GCTCCGT63050.033.894941
CTCCGTT76900.033.2652552
CGGGGAT132150.032.957868
CCGTTTC91250.032.8468634
CGTTTCC84900.032.2879035
TCCGTTT93650.031.5163753
CCCCCGT42600.031.4746651
CGGTTCA127600.031.4535475
TCGGGGA139450.031.3966037
ATAGCGC133600.031.27848294-95
TGTCGGG29200.030.5613445
CCCGTTT61400.030.1835963
Produced by FastQC (version 0.11.5)