FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19777326
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC568670.2875363433863607No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC380740.192513386288925No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT270120.13658064796019442No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC251600.12721638911145017No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT211650.10701648948902394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA62200.063.2172164
GCGACAT67000.059.1134955
CTGCGAC70800.055.6724323
GCTGCGA78550.050.2400672
CGACATC83950.047.2346956
GGCTGCG106500.038.606141
ACTCGGG164600.033.652351
CTCGGGA177850.031.134372
CTAGGCG205900.029.2685137
TCGGGAG192000.028.7408923
TAGGCGG215050.028.1793378
GCTAGGC230200.026.4677316
TGGCTAG249000.024.8889484
TTGATCG80850.024.68503622-23
GGCTAGG254350.024.3841045
TGATCGC83600.023.84472724-25
TCGCCAG89700.022.37593526-27
GGCTGGC282700.021.7821481
GACATCT187500.021.4778087
CCGATAG99550.021.30734490-91
Produced by FastQC (version 0.11.5)