FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23994157
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1206940.5030141296483139No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC961230.400610031850671No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC647280.26976567670204044No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT544980.22713029676349952No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT526840.21957012284282376No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA316760.13201547360051033No Hit
GAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTAT287770.1199333654439287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA129650.075.4493264
GCGACAT134400.072.829975
CTGCGAC142450.068.836583
CGACATC152750.064.0111546
GCTGCGA156050.063.0069122
GGCTGCG188100.052.5508271
ACTCGGG299050.039.7062261
GACATCT256150.038.3942577
CTCGGGA317250.037.439362
ACATCTG272000.036.122038
TCGGGAG336250.035.297083
TTGATCG153700.032.12026622-23
CATCTGT311300.031.6380169
TGATCGC156150.031.37392424-25
TGGCTAG449500.030.7290024
CTAGGCG444550.030.5724017
TAGGCGG459350.029.608058
GATCGCC169400.029.07414624-25
GCTAGGC472650.028.7849526
GGCTGGC482950.028.4200571
Produced by FastQC (version 0.11.5)