FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19958579
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA419270.2100700656093803No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG389690.19524937121024497No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG215760.10810388855839886No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG214040.10724210375899007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC107900.047.8496139
TGTCGGG52050.046.9536675
TTCCGAC113550.045.587238
CTGTCGG59800.040.1606754
CGGGGAT288000.039.9672168
TCGGGGA294650.039.0811967
TCACCCC391950.037.5804569
GTCGGGG69800.036.571096
CCCCGTT58450.036.535712
TTCACCC408950.036.0360228
TTTCCGA146700.035.512137
TCTGTCG68400.035.1112333
GTTCACC430000.034.172547
GTTTCCG128700.033.5116966
CCGTTTC134300.033.1142734
GTTCCGT32350.032.858771
ATAGCGC249300.032.80425394-95
CCCCCGT41550.032.5500261
CGTTTCC135050.031.7655835
ACCGGTT23850.031.6417433
Produced by FastQC (version 0.11.5)