FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21993498
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1880370.8549663177726435No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC684940.311428404885844No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC655540.29806081779260396No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA378220.1719690064763686No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT329370.14975789662926745No Hit
GACCCAGGTGCTACACAGAAGTGGATTCAGTGAATCTAGGAAGACAGCAG275270.1251597176583734No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT115800.066.6838155
TGCGACA116300.066.557724
CTGCGAC127200.061.0788273
CGACATC129550.059.7090726
GCTGCGA130500.059.537852
GGCTGCG169000.046.504561
TGGCTAG506700.042.798934
CTAGGCG514800.041.683147
GCTAGGC533950.040.3393676
TAGGCGG533300.040.246078
GGCTGGC544600.039.482771
GGCTAGG553700.039.1608055
CTGGCTA563050.038.7269063
GCTGGCT576750.037.502422
GACATCT222200.034.8551187
AGGCGGG660950.032.5666969
ACATCTG246650.031.4384928
TTGATCG136050.028.50395622-23
TGATCGC140850.027.48465224-25
CATCTGT286650.026.9852099
Produced by FastQC (version 0.11.5)