FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21993498
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG545710.24812333172285736No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA448540.20394209234019983No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG286990.13048856530234526No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG63950.054.996775
TCCGACC69700.045.4686939
CTGTCGG76550.045.4476974
GTCGGGG86650.042.615236
TTCCGAC77850.040.957068
TCGGGGA379550.040.490377
CGGGGAT380650.040.1471868
TCTGTCG90150.037.8533863
CCCCGTT70450.032.651972
CCCCCGT58300.032.201751
CGCGAAC10050.032.157995
TTTCCGA100600.032.1211937
GTCCCGT30900.031.9930631
ATCGGGG286400.031.8264336
CCGTTTC110150.030.7645854
GTTCCGT30500.030.6985151
CCCGTTA32850.030.527633
CCGATCG120750.030.23058786-87
CCGTTAT25900.030.0947064
CCGTTAA25300.030.056994
Produced by FastQC (version 0.11.5)