FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19318019
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC791860.4099074547964778No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC483450.25025857982643046No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC341360.1767054893154417No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT253630.13129193008869078No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA207060.10718490337958567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA79800.062.9716454
GCGACAT83900.060.0139585
CTGCGAC91100.055.3692253
GCTGCGA100950.050.1548772
CGACATC101050.049.8755236
GGCTGCG137050.037.8245051
CTAGGCG276950.031.4047497
TAGGCGG283600.030.6683548
TGGCTAG290850.030.5702554
GCTAGGC288650.030.197636
GGCTAGG300900.029.4260565
TCGACCG16650.028.8117353
CTGGCTA342300.025.9614663
GCGCGGT68950.025.697036
ACTCGGG188750.025.4250581
GGCTGGC346200.025.3631061
GACATCT202250.025.2245887
ACATCTG206150.024.8395548
TTGATCG103350.024.7024722-23
GCTGGCT364000.024.2832762
Produced by FastQC (version 0.11.5)