FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19318019
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG297100.1537942373904902No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA229060.11857323465723892No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG203410.10529547569033863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC90000.051.603389
TTCCGAC97600.047.779588
GTTCCGT33050.038.5013961
CGGGGAT215550.038.311778
TTTCCGA122750.038.2225347
TGTCGGG45800.038.055385
GTTTCCG109300.037.4516646
TCGGGGA225900.036.6618427
CGTTTCC114500.035.836355
CCGTTTC120200.035.6780434
CCGTTAA22350.035.273484
TCACCCC312950.033.9751139
CTCCGTT94700.033.79282
TCCGTTT124250.033.482043
CCCGTTA24700.033.0701183
GTCCCGT33600.033.0666161
ATAGCGC177850.032.57663794-95
CCCCGTT59250.032.5350882
GCTCCGT88050.031.8152851
TTCACCC336350.031.6113688
Produced by FastQC (version 0.11.5)