FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23412288
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAC85650.044.1694158
TCCGACC89750.042.1006779
CAAACGA99400.038.51096320-21
TCAAACG104900.038.40073820-21
TTTCCGA112250.034.150827
CGGGGAT137550.033.8556148
GTCCCGT34250.033.2668271
ATAGCGC148850.032.87710694-95
GTTCCGT38750.032.1195761
GTTTCCG99550.032.0283856
CCGTTTC120150.031.3805264
GCCCCGT52150.031.2369251
CCCCGTT67650.031.1827262
TCACCCC291750.031.1708779
CGTTTCC109900.031.1460155
CGATCGG157050.031.10614486-87
GCTCCGT83200.031.0190411
TCGGGGA152850.030.8557937
CCGATCG162700.030.53175486-87
ACCCGAT165200.030.15299684-85
Produced by FastQC (version 0.11.5)