FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25825219
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG367670.14236858940092628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG51650.042.3324935
TCCGACC77100.041.4793939
CGGGGAT263200.039.690148
TTCCGAC81150.039.64988
TCGGGGA271650.038.7179647
CTGTCGG63700.034.0260664
CCCCGTT74600.033.8965722
CCCGTTA33800.031.641143
ATCGGGG198300.031.6378066
TTTCCGA105900.031.1910277
GCCCCGT56150.030.8978711
TATCGGG164100.030.674235
GTCCCGT38800.030.1362251
CCGTTTA44350.030.1161174
ATAGCGC144200.030.1053694-95
TCTATCG149000.029.795243
CCGTTAA31400.029.366924
CCGATCG151850.029.3065186-87
GTCGGGG77900.029.224916
CTCCGTT92850.029.1281742
Produced by FastQC (version 0.11.5)