FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16713688
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC915110.5475212891373825No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC600520.35929831883902585No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC205410.1228992667566847No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT169180.10122242320186903No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA79600.072.315654
GCGACAT81350.070.760425
CTGCGAC84350.068.455283
GCTGCGA91200.063.4116362
CGACATC93000.061.8983846
GGCTGCG111000.052.647261
CTAGGCG229450.040.7301757
TAGGCGG231950.040.2911768
GGTCGAA116700.037.9429870-71
GGACGAC120850.037.7848278-79
GCTAGGC249350.037.7660526
GTCGAAG120700.037.6973170-71
ACCCGAC27350.036.9301153
TCGGTCG169550.036.35362268-69
TGGCTAG262700.036.32184
GGCTAGG267300.035.6635065
AGGACGA125700.035.38806578-79
GACATCT164450.035.086717
GAGGACG132300.034.59639776-77
CGAAGAG132950.034.35996272-73
Produced by FastQC (version 0.11.5)