FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21126592
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC374890.17744934914254037No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC303660.14373354680205874No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA51250.059.501634
GCGACAT52350.058.347615
CTGCGAC55350.055.09413
CGACATC60200.050.8141026
GCTGCGA63100.048.7790532
GGCTGCG85800.036.3880731
ACTCGGG150500.032.553911
CTCGGGA160600.030.7296582
TCGGGAG175200.028.0332953
CTAGGCG146100.027.0500477
TAGGCGG152500.026.1017178
TTGATCG63850.024.47352422-23
TGATCGC67050.022.7740424-25
GCTAGGC178400.022.3655366
GGTCGAC42400.022.2362116-17
TCGCCAG69300.022.20596726-27
GATCGCC72350.021.36831924-25
ATTCGGC74100.020.70406338-39
GGCTAGG205800.020.4280415
ACGGGTG36000.020.3191684
Produced by FastQC (version 0.11.5)