FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15917286
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC920500.5783021050196623No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC578790.3636235473811302No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC201800.12678040716237682No Hit
TGGGGGGGATTCTTCTCTAATCTTTCAGAAACTTTGTCTGCGAACACTCT161000.10114789669545424No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA75800.077.1914444
GCGACAT76850.076.136785
CTGCGAC81650.072.009883
GCTGCGA88700.066.7683262
CGACATC91950.063.6852726
GGCTGCG108050.055.4630471
CTAGGCG215150.041.6541447
TAGGCGG218450.041.0031558
GCTAGGC236800.038.0263186
ACCCGAC27950.037.5524373
GACATCT158000.037.483237
TGGCTAG249150.036.732334
GGCTAGG254300.035.9884385
CCGACTC29700.035.499665
CCCGACT31250.034.8027154
TTGATCG86200.034.115722-23
ACATCTG177100.033.279818
ATTCGGC89100.032.91602738-39
GGCTGGC281400.032.8663521
TCGCCAG90150.032.749326-27
Produced by FastQC (version 0.11.5)