FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15279329
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1372910.898540767071643No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC552170.36138367070962346No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC244630.1601051983369165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA76100.069.234094
GCGACAT77800.067.721935
CTGCGAC83800.063.29913
GCTGCGA92450.057.424922
CGACATC94950.055.5851176
CTAGGCG289650.047.458927
TAGGCGG294900.046.5381058
GGCTGCG117300.046.0094951
GCTAGGC304350.045.3720936
TGGCTAG310650.044.9109654
GGCTAGG315450.044.0721865
GGACGAC166500.042.5248978-79
GTCGAAG168400.041.52604770-71
GGTCGAA166850.041.26033870-71
GGCTGGC352650.040.205971
AGGACGA174850.040.05225478-79
CTGGCTA353400.039.8069573
CGAAGAG179650.039.48077872-73
GAGGACG181550.039.2283476-77
TCGGTCG236300.038.46191468-69
Produced by FastQC (version 0.11.5)