FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14547642
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG333320.2291230427584072No Hit
GGAGTCTTGGAAGCTTGACTNCCCTACGTTCTCCTACAAATGGACCTTGA186300.12806199107731686No Hit
CCTCTGTCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG174110.11968262622904798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG36950.047.9229625
TCCGACC50550.042.6807569
CGGGGAT210650.039.672858
TTCCGAC54400.039.6602868
CTGTCGG44200.039.162554
TCGGGGA215750.038.8370867
GTCGGGG48300.038.5613256
TGGTCGT217700.037.99791714-15
TCTGTCG50600.033.599513
GTCCCGT23950.033.2883031
ATCGGGG159300.032.007486
GCCCCGT31000.031.1172371
CCCGTTA18000.030.831473
TTTCCGA71400.030.7107077
CCCCGTT41850.030.4176392
TCTATCG122150.030.3969693
CCCGTTT52800.030.3643283
CCGTTTA25550.030.1680934
CTCCGTT58050.030.1239132
TATCGGG135600.029.9504475
Produced by FastQC (version 0.11.5)