FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21772966
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG401840.18455914550181174No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG400290.18384725351612638No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA321310.14757291220681645No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA318140.14611697827480188No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG219290.10071664099415763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC183850.052.9022649
TTCCGAC184400.052.7535748
TTTCCGA212750.046.0821347
TGTCGGG52700.044.3735735
GTTTCCG193100.043.245056
TCACCCC646500.042.1487739
CCGTTTC189150.041.5622524
CGTTTCC192900.041.468835
GTTCCGT41650.041.4250031
CGGGGAT289000.039.5633248
TCGGGGA299300.038.4406747
CTGTCGG60050.038.3091244
ACCGGTT28950.037.4331323
GTTCACC745550.036.498717
TCCGTTT189000.036.464993
TTCACCC750100.036.2006238
GACGGTT25650.036.1340943
GTCCCGT35900.036.0118451
CTCCGTT152200.035.1011662
ATAGCGC445950.033.61807694-95
Produced by FastQC (version 0.11.5)