FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17614054
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC515750.2928059605131221No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC268290.15231587231423271No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC268110.15221368118889608No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT236660.13435862067869214No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA53850.050.4603774
GCGACAT55800.048.6973885
CTGCGAC64700.042.136473
GCTGCGA68550.040.030732
CGACATC76200.035.7193536
TCAGACG37050.032.931445
TCGACCG17100.031.6243693
GGCTGCG95000.029.747841
GCTCGCG19250.028.3251089
CAGACGT44150.028.0407926
GCGCGGT58700.027.0290936
CTAGGCG194850.026.5843147
TAGGCGG202050.025.614878
TGGCTAG216350.024.6236234
GCTAGGC211000.024.613026
GGCTAGG222350.023.6176685
CGATGCG20450.023.1656842
ACTCGGG162250.022.8195781
CCGCTCG23650.022.6773647
CGCGGTG71600.022.2842337
Produced by FastQC (version 0.11.5)