FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19760163
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG413970.20949725971390015No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG313050.15842480651601912No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA238860.12087956966751742No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA236310.11958909448267203No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG213540.10806591018505261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC137550.054.309929
TTCCGAC138300.054.1950348
TGTCGGG49650.053.3232655
TTTCCGA163450.046.1177647
CTGTCGG60400.043.518014
GTTTCCG149800.043.118996
CGGGGAT282500.041.690098
TCGGGGA286350.041.312147
TCACCCC475400.040.4049
CGTTTCC151800.040.3615
CCGTTTC148750.040.0701874
GTCGGGG68500.040.0355876
GTTCCGT37100.039.2035181
TCCGTTT144600.038.5905153
CTCCGTT118350.036.9483342
GCTCCGT105000.036.5762861
GTTCACC527400.036.569747
GACGGTT23750.036.2239463
ATAGCGC294050.035.67420694-95
TTCACCC546300.035.330668
Produced by FastQC (version 0.11.5)