FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21901747
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1035700.47288465162162635No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC494960.22599110472785572No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC409180.18682527928023276No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT311900.14240873113911873No Hit
AGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCCCGAA308140.14069197311063816No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT275380.12573426220291925No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG237980.1086579988345222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA89600.062.391684
GCGACAT90950.061.5234265
CTGCGAC103500.054.241963
CGACATC113500.049.2163476
GCTGCGA116050.048.9081232
GGCTGCG150800.038.3440671
CTAGGCG350200.032.593847
TGGCTAG356800.032.44064
TAGGCGG367600.031.115658
GCTAGGC369250.031.0666566
GGCTAGG378400.030.5037385
CTGGCTA418600.027.9462433
GGCTGGC416900.027.8761961
ACATCTG216300.026.4403888
ACTCGGG238300.026.3781761
GCTGGCT442450.026.1177732
TCGACCG22500.025.9645523
GACATCT218600.025.9014457
GCGCGGT90650.025.5711296
CTCGGGA256150.024.3645972
Produced by FastQC (version 0.11.5)