FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21901747
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG420550.1920166459780583No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA256000.11688565300293169No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG220580.10071342710697918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC99350.048.562469
TTCCGAC107150.045.2046248
TGTCGGG57650.041.7652635
CGGGGAT288450.041.7167938
TCGGGGA299600.040.2121667
CTGTCGG61800.038.11564
TCACCCC377250.036.592389
TTTCCGA141350.034.9394727
ATCGGGG210750.034.114356
TTCACCC407700.033.9059838
GTCGGGG75350.033.401926
TCTGTCG69400.033.2561873
CCGTTTC132300.033.2040824
CCCCGTT66350.032.989622
GTTTCCG127700.032.4268236
GTTCACC427650.032.1246767
TCTATCG167950.031.5841263
ATAGCGC236600.031.36309294-95
CGTTTCC130150.031.234625
CTATCGG174650.031.2164044
Produced by FastQC (version 0.11.5)