FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18404498
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1030500.5599174723483358No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC794350.43160644751082045No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC301220.1636665123927857No Hit
CAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGA212190.1152924681781595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA103150.074.023254
GCGACAT104900.072.830965
CTGCGAC113300.067.668053
GCTGCGA118250.065.1000752
CGACATC121650.062.8307346
GGCTGCG149900.051.9448551
GACATCT204000.037.8837477
ACATCTG208550.036.9929288
CTAGGCG273400.036.5398837
TAGGCGG281300.035.609048
TGGCTAG294750.034.4894374
GCTAGGC291550.034.4031266
GTCGAAG150250.033.81049370-71
GGCTAGG304500.033.326375
ATTCGGC115850.033.1419638-39
GGACGAC156250.032.9401178-79
GGTCGAA150750.032.85664770-71
TCGGTCG202700.032.6088668-69
TGATTCG118150.032.59101536-37
ACCCGAC29400.032.3878253
Produced by FastQC (version 0.11.5)