FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18404498
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG280710.15252249748947241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG43850.039.6238825
CGGGGAT207550.039.4600878
TCGGGGA216300.037.841877
GTGCTCT185700.036.998469
GGTGTGC142900.036.9245226
CTGTCGG48550.035.5919464
CCCCGTT48700.034.112882
CGGTGTG55050.034.0631875
GTCGGGG56700.033.2238356
TGTGCTC210750.032.6467868
CCGATCT110650.030.85233916-17
GTCCCGT29400.030.846171
TCCGATC105700.030.7260816-17
ATCGGGG157950.030.0115366
GCCCCGT36950.029.9403381
CCCCCGT37000.029.7715511
CTATCGG118350.029.3617824
TCTGTCG58450.029.15653
GTTCCGT25250.029.146441
CCGTTTA30250.028.875674
Produced by FastQC (version 0.11.5)