FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8977960
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT785830.8752879273242473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351320.3913138396695909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311000.34640386011967084No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157410.1753293621268083No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG139620.15551417025694034No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA115510.12865951730682693No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC103330.11509296098445526No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC98720.1099581642154788No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG92820.10338651542221172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91500.090.030161
GTATCAA305250.073.1805951
ATCAACG379850.058.2413833
CAACGCA380950.058.0107155
TCAACGC382400.057.790754
AACGCAG401900.055.001076
ACGCAGA432550.051.1006247
CGCAGAG433600.050.99068
TATCAAC457150.048.5519562
GCAGAGT470600.046.994219
GCAGCGC13750.046.3004078
TGCAGCG14600.043.197317
AGAGTAC511250.031.71296710-11
CAGAGTA495450.030.7728510-11
TGGTCCT40350.029.7960785
GTACTTT627650.029.4548591
TCTGTCG9200.029.1023718
TCGACGC850.007202065528.0086614
GTCGTGC8100.027.188781
AGTACTT511600.026.7544112-13
Produced by FastQC (version 0.11.5)