FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17638971
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC2658861.5073781798269297No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC934480.5297814708125548No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC236570.13411780086264669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA118800.070.790614
GCGACAT120400.069.924145
CTGCGAC134250.062.8772353
GCTGCGA139500.060.6070442
CGACATC146700.057.412936
CTAGGCG468450.054.18657
TAGGCGG476250.053.2990388
GCTAGGC483800.052.568926
TGGCTAG486350.052.454914
GGTCGAA239250.051.9635370-71
GGCTAGG491050.051.9346355
GTCGAAG245150.051.0816270-71
GGACGAC257550.049.23547778-79
GGCTGCG174750.048.9413571
CGAAGAG261800.048.38613572-73
CTGGCTA532850.048.2384223
AGGACGA265550.047.3249378-79
GGCTGGC554600.046.7308731
TCGAAGA267800.046.4920372-73
AGGCGGG549850.046.2704059
Produced by FastQC (version 0.11.5)