FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17638971
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG584080.3311304270526892No Hit
CCTCTGTCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG292910.16605843957677577No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA238890.13543307033046315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG59600.054.0027965
CTGTCGG68800.045.8831984
GTCGGGG77350.044.4758646
TCTGTCG73350.042.1288073
CGGGGAT353600.041.8281638
TCCGACC66300.041.5807279
TCGGGGA361150.041.019427
TTCCGAC67350.041.004328
ATCGGGG261750.033.7551236
GTTCCGT28650.031.818771
TTTCCGA91250.030.9925737
GGGGATG481450.030.9265299
TATCGGG222500.030.7444535
CCGTTTC92700.030.67384
CTATCGG213750.030.3142624
TCTATCG206250.030.310293
ATAGCGC103950.029.5161594-95
TAGTAGC119150.029.470127
CATCGGG46300.029.4261685
GTCCCGT30300.029.3025741
Produced by FastQC (version 0.11.5)