FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12309101
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1911571.552972877548084No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC654480.531704143137667No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC171470.13930343085169258No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG123930.10068160136146417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT85750.076.372745
TGCGACA86150.076.183514
CTGCGAC97700.067.420213
GCTGCGA101700.064.721792
CGACATC109350.059.8030556
CTAGGCG338300.056.166167
TAGGCGG341250.055.6249588
GCTAGGC346250.054.9314356
TGGCTAG349150.054.6656044
GGCTAGG351550.054.3869745
GGCTGCG124750.053.4082951
CTGGCTA381700.050.314973
GGCTGGC400600.048.3390351
AGGCGGG393750.048.244489
GCTGGCT406800.047.163782
GACATCT154750.042.687897
ACATCTG162650.040.3225368
CATCTGT180550.036.5616269
ACCCGAC31900.035.7296523
CGGTCGA264600.035.65534668-69
Produced by FastQC (version 0.11.5)