FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12309101
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG412600.33519913436407744No Hit
CCTCTGTCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG204940.16649469364172087No Hit
GGAGTCTTGGAAGCTTGACTNCCCTACGTTCTCCTACAAATGGACCTTGA169930.1380523240486856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG40500.051.9856455
GTCGGGG52200.042.515126
CTGTCGG48550.042.186924
CGGGGAT248950.040.82328
TCGGGGA251450.040.627477
TCTGTCG50400.039.7637983
TCCGACC41150.039.0571989
TGGTCGT260500.038.85394714-15
TTCCGAC43750.037.1391268
ATCGGGG182500.034.1651846
TCTATCG140100.031.6905273
TATCGGG155350.031.4151465
CTATCGG149950.030.696154
GGGGATG335750.030.452599
AAACGAG49150.030.34301822-23
CCCCCGT28800.030.2824041
TCGACCG204500.029.10972826-27
GTTCCGT21000.028.9452571
CATCGGG30050.028.5825335
TAGTAGC76150.028.4497597
Produced by FastQC (version 0.11.5)