FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20821490
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC316590.15204963717774278No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC304310.1461518844232569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA57450.054.3165174
GCGACAT60550.051.9362145
CTGCGAC70200.044.8572853
GCTGCGA74800.042.0351832
CGACATC95250.032.9657486
GGCTGCG108950.029.833951
ACCCGAC45650.028.6120173
ACTCGGG148450.027.2095031
CTCGGGA163900.024.4869272
CCCGACT53600.024.456874
CCGACTC54200.023.4012175
GCGCGGT45950.023.1573546
TCGGGAG177400.022.8644523
CTAGGCG152100.021.7998057
TTGATCG73500.021.64833322-23
AACCCGA62100.021.4152432
CGACTCC59350.021.2905736
TAGGCGG157500.020.9920648
TCGATCG30300.020.9277916-17
TGATCGC76750.020.7002424-25
Produced by FastQC (version 0.11.5)