FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18396690
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC1693770.9206927985414768No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC579140.3148066309754635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA80200.069.804484
GCGACAT81450.068.78815
CTGCGAC86850.064.87173
CGACATC91500.061.4226536
GCTGCGA95600.058.8873942
CTAGGCG313600.053.179867
TAGGCGG318750.052.3767438
GGCTGCG110500.051.7783431
GGACGAC165750.049.6660678-79
GCTAGGC337550.049.645046
GGTCGAA163300.049.5368770-71
GTCGAAG167250.048.93342670-71
GGCTAGG352100.047.8644945
TGGCTAG355350.047.6783944
AGGACGA176450.046.67996678-79
GAGGACG180500.045.70616576-77
CGAAGAG185200.044.4362572-73
TCGAAGA183200.044.1825272-73
TCGGTCG248100.044.1344668-69
GGCTGGC391350.043.699741
Produced by FastQC (version 0.11.5)