FastQCFastQC Report
Wed 11 May 2022
EGAF00004913502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28255505
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGT4690591.6600623489121855No Hit
AAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTG4007411.4182758368678954No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC3573441.264688066980222No Hit
CCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGG3143581.1125548808984302No Hit
AGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGAC3061761.0835976918480132No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCT2235710.7912475816659443No Hit
GGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGG2018080.7142254226211847No Hit
TGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCA1524870.539671826782073No Hit
GCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCC1304090.4615348407328058No Hit
GTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCA1302110.46083409232997247No Hit
AGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGAT1085600.38420831622014895No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT1076800.3810938788742229No Hit
CAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGT1051280.3720620105710374No Hit
GCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCCGG1046900.37051187016476966No Hit
CATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCA731560.2589088391801881No Hit
CGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACC718160.25416640049434613No Hit
CCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGA710740.2515403635503949No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC703490.24897449187335352No Hit
GAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCG697040.24669175086412368No Hit
CCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCG650420.23019231119741093No Hit
TGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTG635470.2249013068426843No Hit
ACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGC579680.2051564818961827No Hit
AAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCAT558990.19783401499990885No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT556560.19697400559643155No Hit
TGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCG552440.19551588265720254No Hit
TGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTG528660.18709982355650695No Hit
AGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTA518780.18360315980903544No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA510270.18059135733019108No Hit
GCCCCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGG505280.17882532978971707No Hit
AAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC486530.17218945476288602No Hit
GCTTACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCAC479720.169779304953141No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC470180.16640297173948934No Hit
CGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAA445420.1576400775707247No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC443890.15709859016853528No Hit
CAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTG443380.1569180943678055No Hit
GACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGG431620.15275607355097706No Hit
CCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTT416900.14754646926324622No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC414260.1466121380594684No Hit
CCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGC393500.13926489722976107No Hit
GCCCTTCACAAAGAAAAGAGAACTCTCCCCGGGGCTCCCGCCGGCTTCTC384050.1359204162162382No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC371420.13145049079816482No Hit
CCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCGTTA356840.12629043437730098No Hit
TCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGC353130.12497741590532535No Hit
TACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACG350220.12394752810116116No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA350180.1239333715677706No Hit
AACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCACCACCACCCA349250.12360423216643977No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCG346210.12252833562875623No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG338240.11970764635068458No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG334020.11821413207797915No Hit
GCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGAGCGTCGGCAT332710.11775050560943788No Hit
GGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCAT323420.11446265072947731No Hit
TAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGA323070.1143387810623098No Hit
GCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGA312330.11053775184694098No Hit
CAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAAGCCTTCTCC311080.11009536017848556No Hit
GAGGGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTAC306160.10835410657144509No Hit
CCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC302420.10703047069942655No Hit
TACCAAAAGTGGCCCACTAGGCACTCGCATTCCACGCCCGGCTCCACGCC296070.1047831210236731No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA293220.10377446801959476No Hit
GTAGTAGCGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAA289570.102482684347705No Hit
ATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCA286610.10143510087680259No Hit
GATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCA283310.10026718687208033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)