FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12838296
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG288270.22453914444720702No Hit
CCTCTGTCGGGGATGGTCGTNCTCTTCGACCGAGCGCGCAGCTTCGGGAG152000.11839577464174374No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGG33250.046.2313775
CGGGGAT172200.041.0159078
CTGTCGG36550.040.9731754
TCGGGGA174150.040.6324967
GTCGGGG42100.039.3266376
TGGTCGT180600.039.02606614-15
GTCCCGT18800.038.4241521
ATCGGGG127150.033.0379526
CCCCGTT29800.032.8161162
TCCGACC22500.032.6823549
GCCCCGT24450.032.067081
TCTGTCG45900.031.9550633
TCTATCG98000.031.5514373
CCCGTTA16550.031.4045183
TATCGGG108800.031.1720165
CTATCGG103400.031.0964244
GGGGATG239400.029.7048789
CCCCCGT26350.029.4205171
CCGTTAA13950.029.364114
CCCCGAA27350.028.8301012
Produced by FastQC (version 0.11.5)