FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18303980
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC457500.2499456402378062No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC405640.2216130043848387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA42200.034.538224
GCGACAT45500.031.8367275
CTGCGAC50750.028.895663
GCTGCGA57350.025.8820972
CTAGGCG189800.023.5793953
TAGGCGG199200.022.4218244
GCTAGGC210050.021.4764862
TGGCTAG195900.021.3160764
CGACATC68400.021.3073866
GGTGTGC123700.020.8894427
GGCTAGG219000.020.8733731
GCGCGGT50500.020.2727576
ACCCGAC38900.020.228233
GGACGAC125950.019.93232574-75
GGCTGCG80100.019.3768351
GGCGGGT229450.019.3477236
ACGACCA132850.018.97685676-77
GTGTGCT197950.018.7904228
GACGACC133800.018.70656476-77
CGACCAT130150.018.65033578-79
Produced by FastQC (version 0.11.5)