FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18303980
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG240630.13146321182606188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT161700.038.6467258
TCGGGGA165550.037.7193037
TTCCGAC51900.037.484658
TGTCGGG35050.036.5703245
TCCGACC54400.035.7610329
GTCCCGT29050.031.7089841
ATCGGGG120050.031.6993486
TATCGGG97400.030.804295
CTATCGG93800.030.366894
TCTATCG90650.030.0593343
CCCGTTA24750.028.9625643
CCCCCGT37450.028.7806641
CCGATCG103200.028.62666786-87
ATAGCGC97550.028.4357294-95
CCGTTTC82050.028.4089264
GCCCCGT41800.028.2845841
TCACCCC188050.028.2598429
CGATCGG100150.027.69776386-87
GTCGGGG48500.027.5896056
CTGTCGG45800.027.5719854
Produced by FastQC (version 0.11.5)