FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13228680
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC217610.16449864990308935No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATG143170.10822697351512017TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG13850.052.119383
TGCGACA41050.049.283534
GCGACAT40850.049.176065
CCGCTCG17600.041.284237
CTGCGAC49050.041.1486243
CGACATC51300.039.1587146
GCTGCGA52450.038.84342
GCTCGCG19350.038.2867979
CGACCGC20200.036.2055174
CTCGACC22200.032.515922
CTCGCGT12200.030.55731810-11
CGCGTCG12350.030.18652212-13
GGCTGCG70450.029.1845551
CCTCGAC25550.028.6866681
CGTCGTA9300.028.59996438-39
TCGCGTC13150.027.8080410-11
GACCGCT27050.027.5637195
CGTCGCA13600.027.06282614-15
ACCCGAC17250.026.154113
ACGGACC27500.025.903834
Produced by FastQC (version 0.11.5)