FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13228680
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACC26300.037.0069279
TTCCGAC26650.036.5213248
CCCCGTT35750.032.1625672
GTCCCGT20550.032.154441
CGGGGAT51900.030.5482658
CCGTTTA22000.030.4358734
CCGTTAT11300.029.2384
CCCGTTA15850.029.182443
GCCCCGT26100.029.0301671
CCGTTAA15350.028.9853024
CCCCCGT29150.028.712871
TGGTCGT56750.028.47494314-15
GTTCCGT17950.028.4678781
GTCCGTT14550.028.461062
CCCCGAA32850.028.2965372
TCGACCG32150.028.25007626-27
TCGGGGA59000.027.2453427
CCGAATT20150.027.1088734
TCCCCGT36450.026.7089181
TCCGTTA16250.026.5664963
Produced by FastQC (version 0.11.5)