FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004913510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004913510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9064997
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT805990.8891232948008698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355960.39267525405689596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319950.3529510269005053No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161520.17817987143294148No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG142400.15708775193196425No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA118680.1309211685343084No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC104370.11513517323833644No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC103720.11441812942684923No Hit
GGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAG92010.10150030937682605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98750.089.332591
GTATCAA309350.072.1381
ATCAACG389200.057.0132143
CAACGCA393050.056.4396135
TCAACGC394100.056.2590374
AACGCAG414950.053.4870876
ACGCAGA448000.049.5138327
CGCAGAG449200.049.40568
TATCAAC467650.047.5028342
GCAGAGT485200.045.7398959
GCAGCGC13200.040.567268
TGCAGCG14000.037.826027
CGTCAAT5800.036.9326446
AGAGTAC527550.030.95892110-11
CAGAGTA512700.030.29475810-11
GTACTTT645850.029.879971
TGGTCCT37300.029.840445
TCTGTCG10000.026.7743938
TGGTATC29350.026.568192
AGTACTT526150.026.25223412-13
Produced by FastQC (version 0.11.5)